Publications by authors named "E Nosyreva"
The synovial joints senses and responds to a multitude of physical forces to maintain joint homeostasis. Disruption of joint homeostasis results in development of osteoarthritis (OA), a disease characterized by loss of joint space, degeneration of articular cartilage, remodeling of bone and other joint tissues, low-grade inflammation, and pain. How changes in mechanosensing in the joint contribute to OA susceptibility remains elusive.
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- The study investigates the behavior of Piezo1 channels, which open in response to mechanical pressure, focusing on their transition from closed to open states.
- Researchers used advanced pressure-clamp recordings to analyze new subconductance states of Piezo1, revealing that pressure increases the open state while reducing closed states.
- By employing Markov-chain modeling, the team developed a four-state kinetic model that accurately describes the channel's function, aiding future research into Piezo1's role in different cell types.
Article Synopsis
- Prune belly syndrome (PBS), or Eagle-Barret syndrome, is a rare condition mostly affecting males, characterized by urinary tract dilation, abdominal muscle deficiency, and undescended testes.
- Researchers found a genetic link to PBS through a variant in the PIEZO1 gene, which plays a role in how cells respond to mechanical pressure.
- Functional analysis showed that the PIEZO1 mutation leads to loss-of-function in certain channel activities, but the issue can potentially be fixed using Yoda1, a compound that activates PIEZO1, suggesting new treatment avenues for PBS.
Ketamine is a noncompetitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist that exerts rapid antidepressant effects. Preclinical studies identify eukaryotic elongation factor 2 kinase (eEF2K) signaling as essential for the rapid antidepressant action of ketamine. Here, we combine genetic, electrophysiological, and pharmacological strategies to investigate the role of eEF2K in synaptic function and find that acute, but not chronic, inhibition of eEF2K activity induces rapid synaptic scaling in the hippocampus.
View Article and Find Full Text PDFStore-operated calcium entry (SOCE) is activated by depletion of Ca from the endoplasmic reticulum (ER) and mediated by stromal interaction molecule (STIM) proteins. Here, we show that in rat and mouse hippocampal neurons, acute ER Ca depletion increases presynaptic Ca levels and glutamate release through a pathway dependent on STIM2 and the synaptic Ca sensor synaptotagmin-7 (syt7). In contrast, synaptotagmin-1 (syt1) can suppress SOCE-mediated spontaneous release, and STIM2 is required for the increase in spontaneous release seen during syt1 loss of function.
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