Inhibitor binding to metal-substituted metalloenzyme: Sulfonamide affinity for carbonic anhydrase IX.

Transition metal ions are structural and catalytic cofactors of many proteins including human carbonic anhydrase (CA), a Zn-dependent hydrolase. Sulfonamide inhibitors of CA recognize and form a coordination bond with the Zn ion located in the active site of the enzyme. The Zn ion may be removed or substituted with other metal ions.

Enhancing Chromatographic Analysis: A Novel Cryo-Enrichment Module for GC Analysis of Terpenes in Cannabis sativa.

This study presents the development and validation of a gas chromatography (GC) method using cryo-enrichment for the analysis of α-pinene and β-caryophyllene in Cannabis sativa plant. Cryo-enrichment is a method that involves cooling part of the GC column to improve some aspect of chromatographic analysis. The aim of this study was to improve upon previous GC cryo-enrichment prototype and to create and validate a method for the analysis of these terpene compounds, which have received increasing attention for their medicinal properties.

Cheap and easy human breath collection system for trace volatile organic compounds screening using thermal desorption - gas chromatography mass spectrometry.

•By analyzing the VOCs presents in our breath, we could identify if some components should not be present in our bodies, or their concentration is higher or lower than normal.•To collect breath samples for VOC analysis, we looked into the current available methodologies and, due to their high prices, tried to develop our own easy and cheap device. A simple single use Minigrip LDPE plastic bag was used in this work and its efficiency and performance were tested.

Relation between thickness, crystallite size and magnetoresistance of nanostructured La Sr Mn O films for magnetic field sensors.

In the present study the advantageous pulsed-injection metal organic chemical vapour deposition (PI-MOCVD) technique was used for the growth of nanostructured La Sr Mn O (LSMO) films on ceramic AlO substrates. The compositional, structural and magnetoresistive properties of the nanostructured manganite were changed by variation of the processing conditions: precursor solution concentration, supply frequency and number of supply sources during the PI-MOCVD growth process. The results showed that the thick (≈400 nm) nanostructured LSMO films, grown using an additional supply source of precursor solution in an exponentially decreasing manner, exhibit the highest magnetoresistance and the lowest magnetoresistance anisotropy.

Preparation and characterization of quercetin-loaded lipid liquid crystalline systems.

The aim of the present study was to investigate mixtures of soy phosphatidylcholine (SPC) and glycerol dioleate (GDO) as encapsulation matrices for antioxidant quercetin. The effects of quercetin loading into non-aqueous formulations, non-lamellar liquid crystalline phases and their colloidal dispersions were studied by using synchrotron small angle X-ray diffraction, dynamic light scattering, cryogenic electron microscopy and high performance liquid chromatography. Quercetin incorporation is discussed in the context of lipid aggregation behavior, self-assembled nanostructure and chemical stability.