[Interaction of proteins with platinum and palladium compounds with differing biological activities].

Protein interaction with bioactive platinum and palladium complex compounds was studied by solid-phase sorbent assay (SSA), turbidimetry, isoelectric focusing and circular dichroism. In SSA reaction ability of metal compounds can be represented as follows: [equation: see text] Reaction ability of proteins can be represented as follows: hemoglobin > serum albumin > immunoglobulin. The reaction products of serum albumin and metal compounds demonstrated more alkaline isoelectric points then the intact albumin resulting from adding positively charged metal centers [MeCl+, Me2+, [Me(H2O)n]2+, [Me(H2O)nCl]+ to albumin molecules.

[Immunoglobin-binding proteins in human placenta].

Phenomena of the binding of poor-soluble placenta proteins (PSPP) with pregnant women sera IgG as well as placenta blood IgG were studied. PSPP were extracted from the placenta tissue, washed out from soluble proteins, by the use of 3M KCl solution containing 0.005 M PMSF.

[A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes].

The enzyme-linked immunoassay modification has been worked out. The method combines advantages of membrane technology of antigen immobilization which is used in the enzyme immunosensory technique and of conventional enzyme-linked immunosorbent assay. The nitrocellulose and polypropylene membranes are used as a solid-phase.

[Activity of the natural killer cells in the peripheral blood of patients with brain tumors].

Activity of natural killer cells (NK-cells) was determined in a 4-hour 51Cr-release test using K-562 as target cells in patients with brain tumours and donors. This activity was significantly (P less than 0.05) reduced in preoperative patients with metastatic brain tumours (mean--5.

[Proliferative activity of experimental brain tumors].

The method of autoradiography was used to study the parameters of the cell cycle and the intensity of proliferative processes in three transplantable rat brain tumors differing in the duration of the latent period and sensitivity to chemotherapy. The tumors were found to be similar in the duration of the cell cycle and its phases, the mean values of the label index and growth fraction, and were characterized by the irregularity of the proliferative processes within the tumor; the label index was higher in the center of the tumors (threefold in one case) than in the zone of their growth. It is suggested that the sensitivity of brain tumors to chemotherapy does not depend on their cell kinetics.