Extracellular proteolytic enzymes of filamentous fungi.

This review involves generalized data on extracellular proteases of filamentous fungi. General characteristics of secreted proteases and their classification are considered. Analysis of the data available revealed that fungi secrete only serine, aspartyl, and metalloproteases.

The anionic protease inhibitor BWI-1 from buckwheat seeds. Kinetic properties and possible biological role.

Kinetic characteristics and effects on the growth of filamentous fungi of one of the main anionic protease inhibitors, BWI-1, isolated from buckwheat seeds, have been studied. The inhibition constants of bovine trypsin, chymotrypsin and cathepsin G from human granulocytes with BWI-1 were found to be 1.1, 67 and 200 nM, respectively.

Isolation and properties of anionic protease inhibitors from buckwheat seeds.

Three protease inhibitors (BWI-1, BWI-2 and BWI-4) from buckwheat seeds were purified to homogeneity and characterized. Their molecular masses were 7.7-9.