Specific Cytotoxicity of Targeted Lu and Pb-Based Radiopharmaceuticals.

Two radiopharmaceutical preparations were developed on the basis of artificial targeted polypeptide ZHER2 specific to HER2/neu tumor marker and radionuclides Lu (ZHER2-HSA-chelator-Lu) or Pb (ZHER2-HSA-chelator-Pb). The objective was to evaluate in vitro the cytotoxic activity of the targeted radiopharmaceuticals using two cultured human breast cancer cell lines with different expression of HER2/neu: SK-BR3 (high expression of HER2/neu) and MCF-7 (low expression of HER2/neu). It was shown that the cytotoxic effect of both preparations was significantly higher against the SK-BR-3 cells.

Near-Infrared Activated Cyanine Dyes As Agents for Photothermal Therapy and Diagnosis of Tumors.

Today, it has become apparent that innovative treatment methods, including those involving simultaneous diagnosis and therapy, are particularly in demand in modern cancer medicine. The development of nanomedicine offers new ways of increasing the therapeutic index and minimizing side effects. The development of photoactivatable dyes that are effectively absorbed in the first transparency window of biological tissues (700-900 nm) and are capable of fluorescence and heat generation has led to the emergence of phototheranostics, an approach that combines the bioimaging of deep tumors and metastases and their photothermal treatment.

[The activity of enzymes of amino acid metabolism of the placenta in different terms of the physiological and complicated pregnancy.].

The activity of amino acid metabolism enzymes and the content of free amino acids in the placenta during physiological pregnancy and placental insufficiency (PI) were studied using spectrophotometric methods and ion-exchange chromatography. It was found that in PI placental activity of the studied enzymes: alanine-, cysteine-e, tyrosine-, glutamino- transferase, glutathione synthetase, glutamate dehydrogenase decreases at different periods of gestation. The opposite variations occur for aspartataminotranferase and glutaminase.

[Influence of amino acid imbalance in maternal and fetal organisms on the development of placental insufficiency and the course of the neonatal period.].

The content of amino acids in maternal serum and umbilical cord blood during physiological pregnancy and placental insufficiency (PI) was estimated using ion-exchange chromatography. It was found that that the content of arginine, serine, glutamine, alanine, cysteine, methionine, tryptophan, leucine, phenylalanine and proline, which are involved in many metabolic processes necessary to maintain the normal functioning of the mother-placenta-fetus system, was decreases in maternal serum at PI. Opposite deviations have dicarboxylic amino acids, lysine, histidine, glycine, tyrosine and threonine, the number of which increases, strengthening the nitrogen imbalance.

[Targeted Bifunctional Proteins and Hybrid Nanoconstructs for Cancer Diagnostics and Therapies].

In this review, the authors' works published within the past 5 years devoted to the development of bifunctional hybrid nanostructures based on the targeting polypeptides and nanoparticles of various origin (quantum dots, nanogold, nanodiamonds, upconversion nanoparticles, magnetic and polymer nanoparticles) as modules that ensure visualization and various damaging effects on cancer cells are surveyed and the prospects of their application in theranostics and precision medicine have been contemplated.