Cryoglobulin composition in patients with myocardial infarction.

The formation of an ischemic focus in the myocardium is paralleled by autoimmune processes involving immunoglobulins with abnormal thermal solubility, cryoglobulins. Serum levels of cryoglobulins and their complexes were measured in 25 patients with Q-forming myocardial infarction on days 1, 2, 3, 7, and 14 of the disease. Cryoprotein levels were measured by spectrophotometry, concentrations of IgM, IgA, IgG, and fibronectin by ELISA.

[Cryoglobulins in patients with ischemic stroke].

The composition of circulating immune complexes with cryo-properties (cryo-IC) were determined in 70 patients on days 1, 2, 3, 7, 14 and 21 after ischemic carotid stroke. The quantitative and qualitative assessments of cryo-IC content were conducted on a spectrophotometer and on a vertical electrophoresis, respectively. Concentrations of immunoglobulins (Ig) M, A and C, fibronectin, percentage of IgM with rheumatoid factor activity, IgG isotypes (IgG1, IgG2, IgG3, IgG4) were measured with ELISA.

Autologous progenitor cell implantation as a novel therapeutic intervention for ischaemic digits in systemic sclerosis.

Objectives: The effects of local stem cell implantation on clinical and functional characteristics of peripheral vascular disease were studied in two SSc patients with non-healing ischaemic ulcers.

Methods: The local injections of CD34(+) cells from peripheral blood (PB) after mobilization by G-CSF (Case 1) and bone marrow (BM) (Case 2) were used for ischaemic skin ulcers in hands, while mononuclear cells (MNCs) were implanted in lower extremities of the same patients. Ischaemic status was evaluated by measuring ulcer healing, Raynaud's condition score (RCS), visual analogue pain, RP and ulcer scales.

Effect of routine therapy and selective plasmapheresis on electrical properties of cryoglobulins during ischemic stroke.

The charge properties of cryoglobulins were examined during the first 21 days of ischemic stroke (atherothrombotic and cardioembolic). Routine drug therapy produced no effect on the charge of cryoglobulins. Plasmapheresis significantly modified the electrokinetic parameters of cryoglobulins during atherothrombotic stroke and produced less pronounced effect on these proteins during cardioembolic stroke.

Determination of optimal conditions for studies of electrophoretic mobility of human erythrocytes loaded with cryoglobulins.

For studies of the charge characteristics of human erythrocytes loaded with cryoglobulins the cells should be stored in Alsever solution with 0.1% sodium azide no longer than 1 week after blood collection. Erythrocytes should be loaded with cryoglobulins by incubation during at least 4 h, the final concentration of cryoproteins in the solution being no lower than 30 microg/ml.