Publications by authors named "E Mullner"
Storage of packed red blood cells is associated with changes in erythrocytes that over time increasingly impair cellular function and potentially contribute to adverse effects associated with blood transfusion. Exposure of phosphatidylserine at the outer membrane leaflet of erythrocytes and shedding of microvesicles (MVs) during packed red blood cell storage are alterations assumed to increase the risk of prothrombotic events in recipients. Here, we used rotational thromboelastometry to study the coagulation process in blood samples with erythrocytes from stored PRBCs reconstituted with freshly prepared platelet-rich plasma.
View Article and Find Full Text PDFThe mycobacteriophage Pinkcreek (C1 subcluster) was extracted from soil collected on the Dr. Norman C. Francis Parkway Bike Trail in New Orleans, Louisiana.
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- Premature infants often receive adult packed red blood cells (pRBCs), which may not suit their unique physiological needs due to substantial differences between adult and preterm red blood cells.
- The lack of standardised transfusion protocols complicates the comparison of clinical data and obscures the understanding of pRBC transfusion's associations with complications like bronchopulmonary dysplasia and neurodevelopmental impairment.
- To address these challenges, new methods that combine biochemical and clinical research could help establish clearer causal relationships between pRBC transfusions and their complications, enabling better transfusion practices for neonates.
Pantothenate kinase-associated neurodegeneration (PKAN) is a progressive neurodegenerative disease caused by mutations in the pantothenate kinase 2 (PANK2) gene and associated with iron deposition in basal ganglia. Pantothenate kinase isoforms catalyze the first step in coenzyme A (CoA) biosynthesis. Since PANK2 is the only isoform in erythrocytes, these cells are an excellent ex vivo model to study the effect of PANK2 point mutations on expression/stability and activity of the protein as well as on the downstream molecular consequences.
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- Ascorbic acid (AA), or vitamin C, is crucial for certain mammals that cannot produce it themselves, relying instead on their diet for this antioxidant.
- Human red blood cells express the GLUT1 transporter, which allows for quick uptake of glucose and dehydroascorbate (DHA), an oxidized form of AA, enhancing cellular functions like reducing oxidized species.
- The study findings suggest that DHA uptake not only improves the redox balance by decreasing harmful free radicals but also supports the recycling of AA, hinting at evolutionary adaptations in mammals that cannot synthesize AA.