Role of sensory afferents in the myoelectric response to acute enteric inflammation in the rabbit.

The role of sensory afferents in inflammation-induced alterations in myoelectric activity in vivo was investigated in the rabbit small intestine. Isolated ileal loops were implanted with serosal electrodes and exposed to ricin or vehicle after pretreatment with 125 mg/kg of subcutaneous (125 mg over 3 days) or intraluminal (640 microM) capsaicin. After 5 h of myoelectric recording, the loops were prepared for histology and for ex vivo generation of eicosanoids.

IKir regulation in murine macrophages: whole cell and perforated patch studies.

Previous studies have reported that the inwardly rectifying K+ conductance (GKir) in macrophages is modulated by intracellular perfusion with inositol 1,4,5-triphosphate (InsP3), inositol 1,3,4,5-tetrakisphosphate (InsP4), or GTP analogues and by exposing cells to macrophage-specific colony-stimulating factor (CSF) I. This study uses both conventional whole cell and amphotericin B perforated patch studies to investigate GKir modulation in thioglycollate-elicited mouse peritoneal macrophages (MO). Under whole cell recording conditions with 150 mM Cl- in the patch pipette, GKir decreased within 25 min.

Effect of leukotriene D4 on gastric emptying and secretion in rhesus monkeys.

Gastric function was evaluated in conscious rhesus monkeys after an i.v. infusion of saline (0.

Aspirin-induced changes in gastric function: role of endogenous prostaglandins and mucosal damage.

The relative roles of prostaglandins and mucosal injury in aspirin-induced changes in gastric function were evaluated. Conscious rhesus monkeys received a subcutaneous injection of sodium bicarbonate or aspirin (25, 50, 100, or 150 mg/kg) and sodium bicarbonate or 150 mg/kg aspirin subcutaneously plus oral sucralfate (25 mg/kg twice a day). Gastric emptying and fluid and H+ outputs were determined during a fasting period and after an 80-ml water load using a 99mTc-diethylenetriaminepentaacetic acid dilution technique.