Publications by authors named "E Miska"
Article Synopsis
- Long noncoding RNAs (lncRNAs) play crucial roles in mammalian cells, but their functional mechanisms and structural organization are not well understood.
- The study focuses on the NORAD lncRNA, which acts as a decoy for Pumilio proteins involved in regulating gene expression and genomic stability, particularly under stress conditions.
- Through analysis, researchers found that NORAD has a modular structure that allows it to interact with different RNAs and cluster binding sites, enhancing its regulatory function, which can inform the design of artificial lncRNAs for specific purposes.
Article Synopsis
- PIWI-interacting RNAs (piRNAs) are vital for maintaining genome integrity by silencing mobile genetic elements and are derived from long single-stranded precursors in various species.
- The study explores how piRNA clusters form and adapt to genomic threats, presenting a roadmap of piRNA clusters in seven species, highlighting shared traits and differences.
- Findings reveal transcriptional readthrough as a mechanism for piRNA production in mammals, especially in response to retroviral threats, and uncover dynamic piRNA clusters in human germ cells, enhancing understanding of piRNA biology across species.
pre-mRNA splicing is a critical feature of eukaryotic gene expression. Both cis- and trans-splicing rely on accurately recognising splice site sequences by spliceosomal U snRNAs and associated proteins. Spliceosomal snRNAs carry multiple RNA modifications with the potential to affect different stages of pre-mRNA splicing.
View Article and Find Full Text PDFEast African cichlid fishes have diversified in an explosive fashion, but the (epi)genetic basis of the phenotypic diversity of these fishes remains largely unknown. Although transposable elements (TEs) have been associated with phenotypic variation in cichlids, little is known about their transcriptional activity and epigenetic silencing. Here, we describe dynamic patterns of TE expression in African cichlid gonads and during early development.
View Article and Find Full Text PDFSummary: RNA (ribonucleic acid) molecules have secondary and tertiary structures in vivo which play a crucial role in cellular processes such as the regulation of gene expression, RNA processing and localization. The ability to investigate these structures will enhance our understanding of their function and contribute to the diagnosis and treatment of diseases caused by RNA dysregulation. However, there are no mature pipelines or packages for processing and analyzing complex in vivo RNA structural data.
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