Ahf-Caltide, a Novel Polypeptide Derived from Calpastatin, Protects against Oxidative Stress Injury by Stabilizing the Expression of Ca1.2 Calcium Channel.

Reperfusion after ischemia would cause massive myocardial injury, which leads to oxidative stress (OS). Calcium homeostasis imbalance plays an essential role in myocardial OS injury. Ca1.

Regulation of Cardiac Cav1.2 Channels by Calmodulin.

Cav1.2 Ca channels, a type of voltage-gated L-type Ca channel, are ubiquitously expressed, and the predominant Ca channel type, in working cardiac myocytes. Cav1.

Ca Dyshomeostasis Links Risk Factors to Neurodegeneration in Parkinson's Disease.

Parkinson's disease (PD), a common neurodegenerative disease characterized by motor dysfunction, results from the death of dopaminergic neurons in the substantia nigra pars compacta (SNc). Although the precise causes of PD are still unknown, several risk factors for PD have been determined, including aging, genetic mutations, environmental factors, and gender. Currently, the molecular mechanisms underlying risk factor-related neurodegeneration in PD remain elusive.

Properties of Calmodulin Binding to Na1.2 IQ Motif and Its Autism-Associated Mutation R1902C.

Voltage-gated sodium channels (VGSCs) are fundamental to the initiation and propagation of action potentials in excitable cells. Ca/calmodulin (CaM) binds to VGSC type II (Na1.2) isoleucine and glutamine (IQ) motif.

Purification of insoluble GST-fused and GST-cleaved Cav1.2 channel fragment by denaturation and renaturation.

Both recombinant glutathione-S-transferase (GST)-fused and GST-cleaved fragments of an L-type voltage-gated Ca channel (Cav1.2) are used frequently in GST pull-down assays to investigate the interactions between regulatory proteins and the Cav1.2 channel.