TP53 germline testing and hereditary cancer: how somatic events and clinical criteria affect variant detection rate.

Background: Germline heterozygous pathogenic variants (PVs) in TP53 cause Li-Fraumeni syndrome (LFS), a condition associated with increased risk of multiple tumor types. As the associated cancer risks were refined over time, clinical criteria also evolved to optimize diagnostic yield. The implementation of multi-gene panel germline testing in different clinical settings has led to the identification of TP53 PV carriers outside the classic LFS-associated cancer phenotypes, leading to a broader cancer phenotypic redefinition and to the renaming of the condition as "heritable TP53-related cancer syndrome" (hTP53rc).

Improving the Evaluation of Aesthetic Outcomes in DIEP Flap Breast Reconstruction: Validation of the Aesthetic Grading Tool.

Introduction: This study aimed to develop and validate an aesthetic grading tool (AGT) for bilateral DIEP flap breast reconstruction and investigate the correlation of BREAST-Q scores with perceived aesthetic outcomes.

Methods: The AGT utilized a Likert scale to rate aesthetic outcomes based on photographs of post-reconstruction breasts. The validation involved iterative testing with healthcare providers and patients.

High removal of PS and PET microplastics from tap water by using FeO porous microparticles and photothermal irradiation with NIR light.

This work reports the synthesis of FeO (FeO) microparticles using the Pechini method and their use to remove microplastics from tap water. The analysis by electronic microscopy revealed that the FeO microparticles (FeMicroPs) have a porous structure and are formed by interconnected grains with sizes of 80-120 nm. In addition, the X-ray diffraction analysis pointed out that the FeMicroPs are composed of γ- FeO and α- FeO phases.

α,ω-Alkanedibromides Form Low Conductance Chemisorbed Junctions with Silver Electrodes.

Chemical groups capable of connecting molecules physically and electrically between electrodes are of critical importance in molecular-scale electronics, influencing junction conductance, variability, and function. While the development of such linkage chemistries has focused on interactions at gold, the distinct reactivity and electronic structure of other electrode metals provides underexplored opportunities to characterize and exploit new binding motifs. In this work we show that α,ω-alkanedibromides spontaneously form well-defined junctions using silver, but not gold, electrodes through application of the glovebox-based scanning tunneling microscope-based break junction method.

Resolving molecular frontier orbitals in molecular junctions with kHz resolution.

Designing and building single-molecule circuits with tailored functionalities requires a detailed knowledge of the junction electronic structure. The energy of frontier molecular orbitals and their electronic coupling with the electrodes play a key role in determining the conductance of nanoscale molecular circuits. Here, we developed a method for measuring the current-voltage (-) characteristics of single-molecule junctions with a time resolution that is two orders of magnitude higher than previously achieved.