Artifact-free holographic light shaping through moving acousto-optic holograms.

Holographic light modulation is the most efficient method to shape laser light into well-defined patterns and is therefore the means of choice for many intensity demanding applications. During the last two decades, spatial light modulators based on liquid crystals prevailed among several technologies and became the standard tool to shape light holographically. But in the near future, this status might be challenged by acousto-optic deflectors.

Positioning Accuracy in Holographic Optical Traps.

Spatial light modulators (SLMs) have been widely used to achieve dynamic control of optical traps. Often, holographic optical tweezers have been presumed to provide nanometer or sub-nanometer positioning accuracy. It is known that some features concerning the digitalized structure of SLMs cause a loss in steering efficiency of the optical trap, but their effect on trap positioning accuracy has been scarcely analyzed.

Acousto-holographic optical tweezers.

Acousto-optic deflectors (AODs) allow the creation of multiple optical traps by time-sharing, that is, by rapidly cycling the laser focus between designated spatial locations. The traps thus formed are not permanent. In this Letter, we successfully demonstrate the creation of multiple and permanent traps by means of AODs driven by specially encoded radio frequency signals.

Influence of experimental parameters on the laser heating of an optical trap.

In optical tweezers, heating of the sample due to absorption of the laser light is a major concern as temperature plays an important role at microscopic scale. A popular rule of thumb is to consider that, at the typical wavelength of 1064 nm, the focused laser induces a heating rate of B = 1 °C/100 mW. We analysed this effect under different routine experimental conditions and found a remarkable variability in the temperature increase.

Extending calibration-free force measurements to optically-trapped rod-shaped samples.

Optical trapping has become an optimal choice for biological research at the microscale due to its non-invasive performance and accessibility for quantitative studies, especially on the forces involved in biological processes. However, reliable force measurements depend on the calibration of the optical traps, which is different for each experiment and hence requires high control of the local variables, especially of the trapped object geometry. Many biological samples have an elongated, rod-like shape, such as chromosomes, intracellular organelles (e.