Publications by authors named "E Margeat"
Article Synopsis
- The study investigates the structural basis of allosteric interactions in heterodimeric G protein-coupled receptors (GPCRs), specifically focusing on metabotropic glutamate (mGlu) receptors, which are crucial for synaptic regulation.
- Researchers utilized cryo-electron microscopy to reveal four distinct structures of the mGlu2-4 heterodimer, showcasing different activation states, including inactive, intermediate, and active forms.
- Findings indicate that agonist binding to just one subunit isn't enough for full activation of the dimer, highlighting the asymmetric nature of mGlu receptor activation, where only mGlu4 activates G proteins.
Allosteric modulators bear great potential to fine-tune neurotransmitter action. Promising targets are metabotropic glutamate (mGlu) receptors, which are associated with numerous brain diseases. Orthosteric and allosteric ligands act in synergy to control the activity of these multidomain dimeric GPCRs.
View Article and Find Full Text PDFSingle-molecule Förster-resonance energy transfer (smFRET) experiments allow the study of biomolecular structure and dynamics in vitro and in vivo. We performed an international blind study involving 19 laboratories to assess the uncertainty of FRET experiments for proteins with respect to the measured FRET efficiency histograms, determination of distances, and the detection and quantification of structural dynamics. Using two protein systems with distinct conformational changes and dynamics, we obtained an uncertainty of the FRET efficiency ≤0.
View Article and Find Full Text PDFRecA-mediated homologous recombination (HR) is a key mechanism for genome maintenance and plasticity in bacteria. It proceeds through RecA assembly into a dynamic filament on ssDNA, the presynaptic filament, which mediates DNA homology search and ordered DNA strand exchange. Here, we combined structural, single molecule and biochemical approaches to characterize the ATP-dependent assembly mechanism of the presynaptic filament of RecA from Streptococcus pneumoniae (SpRecA), in comparison to the Escherichia coli RecA (EcRecA) paradigm.
View Article and Find Full Text PDFReal-time visualization and quantification of viruses released by a cell are crucial to further decipher infection processes. Kinetics studies at the single-cell level will circumvent the limitations of bulk assays with asynchronous virus replication. We have implemented a "viro-fluidic" method, which combines microfluidics and virology at single-cell and single-virus resolutions.
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