Publications by authors named "E Maffiotte"
Trabectedin, a naturally occurring substance isolated from the Caribbean marine invertebrate Ecteinascidia turbinata, is the active compound of the antitumor drug Yondelis®. The mechanism of action of Trabectedin has been attributed to interactions with the minor groove of the DNA double helix, thereby affecting transcription of different genes involved in DNA repair and thus facilitating lethal DNA strand breaks. Nevertheless, the existence of other clinically important molecular mechanisms has not yet been fully explored.
View Article and Find Full Text PDFMetabolic syndrome in adolescents in the Balearic Islands, a Mediterranean region.
Nutr Metab Cardiovasc Dis
June 2011
Background And Aims: To determine the prevalence of metabolic syndrome (MetS) and its components in adolescents in the Balearic Islands, in the western Mediterranean Sea.
Methods And Results: A cross-sectional nutritional survey was carried out in the Balearic Islands (2007-2008). A random sample (n=362, 143 boys and 219 girls) of the adolescent population (12-17 years) was interviewed, anthropometrically measured, and provided a fasting blood sample.
Article Synopsis
- Researchers developed a multiplex real-time PCR method to quickly detect the V617F mutation in the JAK2 gene along with inherited mutations in factors F5 and F2, linked to thrombotic events.
- The method utilized the LightCycler 2.0 and allowed simultaneous amplification of all three genes in a closed tube system with specific primers and FRET-hybridization probes.
- In a study of 41 samples, 46.34% tested positive for the V617F mutation, with a notable percentage also showing heterozygosity for factors F2 and F5, demonstrating the technique's efficiency and reliability in molecular diagnosis.