Evaluation of Nanopore Sequencing on Polar Bodies for Routine Pre-Implantation Genetic Testing for Aneuploidy.

Background: Preimplantation genetic testing for aneuploidy (PGT-A) using polar body (PB) biopsy offers a clinical benefit by reducing the number of embryo transfers and miscarriage rates but is currently not cost-efficient. Nanopore sequencing technology opens possibilities by providing cost-efficient and fast sequencing results with uncomplicated sample preparation work flows.

Methods: In this comparative experimental study, 102 pooled PB samples (99 passing QC) from 20 patients were analyzed for aneuploidy using nanopore sequencing technology and compared with array comparative genomic hybridization (aCGH) results generated as part of the clinical routine.

Marking parties for marking written assessments: A spontaneous community of practice.

In programmes of assessment with both high and low-stakes assessments, the inclusion of open-ended long answer questions in the high-stakes examination can contribute to driving deeper learning among students. However, in larger institutions, this would generate a seemingly insurmountable marking workload. In this study, we use a focused ethnographic approach to explore how such a marking endeavour can be tackled efficiently and pragmatically.

Impact of maternally derived meiotic aneuploidies on early embryonic development in vitro.

Purpose: To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies.

Methods: This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the genetic results from polar body diagnosis.

Results: Of 930 oocytes examined, 566 (60.