Relocation preference and settlement: Lessons from the Poverty Alleviation Relocation Program in China.

We explored the linkages between socioeconomic and demographic factors, relocation preference, and settlement associated with China's Poverty Alleviation Relocation Program. Using multivariate ordinal logistic regression, panel data modeling, and multilevel methods, we found that outdated infrastructure at places of origin, such as long distances to paved roads or elementary schools, increased the probability of relocation, and the presence of left-behind household members at the origin compromised re-settlement. This study sheds light on the community- and household-level factors that influence relocation preference and settlement, offering valuable insights for future research and informing the design and implementation of relocation projects.

Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase.

Determination of substrate binding affinity (K) is critical to understanding enzyme function. An extensive number of methods have been developed and employed to study ligand/substrate binding, but the best approach depends greatly on the substrate and the enzyme in question. Below we describe how to measure the K of BesD, a non-heme iron halogenase, for its native substrate lysine using equilibrium dialysis coupled with High Performance Liquid Chromatography (HPLC) for subsequent detection.

Observations of enhanced rainfall variability in Kenya, East Africa.

Understanding local patterns of rainfall variability is of great concern in East Africa, where agricultural productivity is dominantly rainfall dependent. However, East African rainfall climatology is influenced by numerous drivers operating at multiple scales, and local patterns of variability are not adequately understood. Here, we show evidence of substantial variability of local rainfall patterns between 1981 and 2021 at the national and county level in Kenya, East Africa.

Controllable multi-halogenation of a non-native substrate by SyrB2 iron halogenase.

Geminal, multi-halogenated functional groups are widespread in natural products and pharmaceuticals, yet no synthetic methodologies exist that enable selective multi-halogenation of unactivated C-H bonds. Biocatalysts are powerful tools for late-stage C-H functionalization, as they operate with high degrees of regio-, chemo-, and stereoselectivity. 2-oxoglutarate (2OG)-dependent non-heme iron halogenases chlorinate and brominate aliphatic C-H bonds offering a solution for achieving these challenging transformations.

Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase.

Determination of substrate binding affinity () is critical to understanding enzyme function. An extensive number of methods have been developed and employed to study ligand/substrate binding, but the best approach depends greatly on the substrate and the enzyme in question. Below we describe how to measure the of BesD, a non-heme iron halogenase, for its native substrate lysine using equilibrium dialysis with subsequent detection with High Performance Liquid Chromatography (HPLC).