A probe-based capture enrichment method for detection of A-to-I editing in low abundance transcripts.

Exactly two decades ago, the ability to use high-throughput RNA sequencing technology to identify sites of editing by ADARs was employed for the first time. Since that time, RNA sequencing has become a standard tool for researchers studying RNA biology and led to the discovery of RNA editing sites present in a multitude of organisms, across tissue types, and in disease. However, transcriptome-wide sequencing is not without limitations.

Terminal complement complexes with or without C9 potentiate antimicrobial activity against .

Unlabelled: The complement cascade is a front-line defense against pathogens. Complement activation generates the membrane attack complex (MAC), a 10-11 nm diameter pore formed by complement proteins C5b through C8 and polymerized C9. The MAC embeds within the outer membrane of Gram-negative bacteria and displays bactericidal activity.

Polarization-resolved transmission matrices of specialty optical fibers.

Transmission matrix measurements of multimode fibers are now routinely performed in numerous laboratories, enabling control of the electric field at the distal end of the fiber and paving the way for the potential application to ultrathin medical endoscopes with high resolution. The same concepts are applicable to other areas, such as space division multiplexing, targeted power delivery, fiber laser performance, and the general study of the mode coupling properties of the fiber. However, the process of building an experimental setup and developing the supporting code to measure the fiber's transmission matrix remains challenging and time consuming, with full details on experimental design, data collection, and supporting algorithms spread over multiple papers or lacking in detail.

High-dimensional spectral flow cytometry of activation and phagocytosis by peripheral human polymorphonuclear leukocytes.

Polymorphonuclear lymphocytes (PMNs) are terminally differentiated phagocytes with pivotal roles in infection, inflammation, tissue injury, and resolution. PMNs can display a breadth of responses to diverse endogenous and exogenous stimuli, making understanding of these innate immune responders vital yet challenging to achieve. Here, we report a 22-color spectral flow cytometry panel to profile primary human PMNs on population and single cell levels for surface marker expression of activation, degranulation, phagocytosis, migration, chemotaxis, and interaction with fluorescently labeled cargo.

Comparative mitogenomic analysis of f. sp. suggests recombination events during its evolutionary history.

Introduction: Modern understanding of the concept of genetic diversity must include the study of both nuclear and organellar DNA, which differ greatly in terms of their structure, organization, gene content and distribution. This study comprises an analysis of the genetic diversity of the smut fungus f. sp.