Monolithic thin-layer chromatography plates with covalently bonded matrix for hyphenation with matrix-assisted laser desorption/ionization.

Monolithic layers prepared by the copolymerization of ethylene dimethacrylate and glycidyl methacrylate and deposited onto the surface of glass and silicon plates were investigated as thin-layer chromatography separation media in hyphenated thin-layer chromatography with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. Varying composition of the polymerization mixture and polymerization conditions layers of different porosities and MALDI-MS compatibility were synthesized. Compatibility with MALDI-MS was tested using polyethylene glycol and it was demonstrated that layers prepared without glycidyl methacrylate are not compatible with MALDI-MS and do not allow obtaining any mass spectra.

Recent advances in the preparation of adsorbent layers for thin-layer chromatography combined with matrix-assisted laser desorption/ionization mass-spectrometric detection.

This review is focused on planar chromatography hyphenated with mass-spectrometric detection for analysis of low-molecular-mass solutes. Various kinds of hyphenations are discussed with attention paid to the preparation of thin layer plates suited both for the mass-spectrometric detection of the resolved solutes direct from thin-layer plates and for indirect mass-spectrometric detection of the resolved solutes, performed by scraping, extracting, purifying, and concentrating the analyte from the thin-layer chromatography plate. Plates with monolithic layers are relatively new for thin-layer chromatography but they can successfully be combined with mass-spectrometric technique in a pursuit of comprehensive local sample composition information.

[The efficacy of the dispensary observation of patients with ischemic heart disease who live in a rural locality].

30 patients with chronic ischemic heart disease allocated to controlled treatment and close monitoring, and 30 patients with ischemic heart disease placed under routine dispensary observation, living in rural areas, were examined. Effectiveness of dispensary follow-up of the IHD patients was found to be dependent upon three factors: use of differentiated approach to prescription of drugs, continuity of work in organization of treatment and feasibility of giving therapy under carefully health monitored conditions.

[Temporary disability among workers in bread-baking plants].

A study of the impact of sanitary and hygienic, curative and preventive measures on the morbidity associated with temporary disability was conducted. Bread-baking plants N degrees 1 and N degrees 2 of the city of Vinnitsa were inspected, the number of workers there being 385 and 486, respectively, during 4 years. At the bread-baking plant N degrees 2, where general reconstruction of the production process had been carried out and special curative and preventive measures were being carried out, temporary disability was compared with analogous indicators in persons working at the bread-baking plant N degrees 1, where working conditions and the type of medical care remained unchanged.