Publications by authors named "E M Kachaylo"
Article Synopsis
- Surgical liver failure (SLF) can occur after surgeries where too much liver tissue is removed, often leading to high mortality rates, but its causes are not well understood.
- Researchers used mouse models of different liver surgeries to investigate the effects of blood flow and oxygen levels on SLF, finding that early hypoxia contributes to the problem.
- They discovered that boosting lipid oxidation through treatments like L-carnitine can improve liver regeneration and survival rates in both mice and patients, suggesting that enhancing lipid oxidation could be a promising strategy to mitigate SLF risks in clinical settings.
Article Synopsis
- The study investigates liver regeneration mechanisms by analyzing 271 intracellular signaling pathways in mice after two types of liver surgeries: normal hepatectomy (removing 68% of the liver) and extended hepatectomy (removing 86%).
- RNA sequencing was used to profile liver tissue at different times post-surgery (1 to 48 hours), aiming to identify how specific intracellular signaling pathways are activated or silenced throughout the regeneration process.
- The findings reveal that successful liver regeneration involves dynamic changes in signal pathway activity, with some pathways being strong activators in normal conditions while others constrain regeneration in more extreme cases.
ALPPS (associating liver partition and portal vein ligation for staged hepatectomy), a novel 2-staged hepatectomy, dramatically accelerates liver regeneration and thus enables extensive liver tumor resection. The signaling networks underlying the ALPPS-induced accelerated regeneration process are largely unknown. We performed transcriptome profiling (TP) of liver tissue obtained from a mouse model of ALPPS, standard hepatectomy (68% model), and additional control surgeries (sham, PVL and Tx).
View Article and Find Full Text PDFThe ability of the liver to restore its original volume following tissue loss has been associated with the Hippo-YAP1 pathway, a key controller of organ size. Yes-associated protein 1 (YAP1)-a growth effector usually restrained by Hippo signaling-is believed to be of particular importance; however, its role in liver regeneration remains ill-defined. To explore its function, we knocked down YAP1 prior to standard 70%-hepatectomy (sHx) using a hepatocyte-specific nanoformulation.
View Article and Find Full Text PDFObjective: To investigate whether exercise improves outcomes of surgery on fatty liver, and whether pharmacological approaches can substitute exercising programs.
Summary Of Background Data: Steatosis is the hepatic manifestation of the metabolic syndrome, and decreases the liver's ability to handle inflammatory stress or to regenerate after tissue loss. Exercise activates adenosine monophosphate-activated kinase (AMPK) and mitigates steatosis; however, its impact on ischemia-reperfusion injury and regeneration is unknown.