FISH-Flow Cytometry Reveals Microbiome-Wide Changes in Post-Translational Modification and Altered Microbial Abundance Among Children with Inflammatory Bowel Disease.

Metaproteomic analysis of microbiome post-translation modifications (PTMm) is challenging, and little is known about the effects of inflammation on the bacterial PTM landscape in IBD. Here, we adapted and optimised fluorescence in situ hybridisation-flow cytometry (FISH-FC) to study microbiome-wide tyrosine phosphorylation (p-Tyr) in children with and without inflammatory bowel disease (IBD). Microbial p-Tyr signal was significantly higher in children with IBD, compared to those without.

Primary sclerosing cholangitis in children with inflammatory bowel disease: An ESPGHAN position paper from the Hepatology Committee and the IBD Porto group.

Objective: We aimed to provide an evidence-supported approach to diagnose, monitor, and treat children with inflammatory bowel disease (IBD) and primary sclerosing cholangitis (PSC).

Methods: The core group formulated seven PICO-structured clinical questions. A systematic literature search from inception to December 2022 was conducted by a medical librarian using MEDLINE and EMBASE.

How often do parents administer medications to their children in hospital? A prospective direct observational study.

Parents and carers play a critical role in supporting their children while in hospital. Multiple qualitative studies have explored parental involvement in the care of hospitalised children. Administration of medication to young children can be difficult and cause anxiety and stress for children.

Sexual satisfaction mediates daily associations between body satisfaction and relationship satisfaction in new parent couples.

One of the most common reported postpartum concerns, linked with relationship dissatisfaction, is how body image will affect sexuality. Outside of the postpartum period, feeling more satisfied with one's body is linked with feeling more sexually and relationally satisfied, with some cross-sectional evidence that sexual satisfaction mediates these associations. This dyadic daily experience study examined how new parents' daily satisfaction with their own and their partner's bodies relates to their own sexual satisfaction, and in turn, their own and their partner's relationship satisfaction during a vulnerable time in their sexual and romantic relationship-3- to 4-months postpartum.