Body-wide chimerism and mosaicism are predominant causes of naturally occurring ABO discrepancies.

Routine ABO blood group typing of apparently healthy individuals sporadically uncovers unexplained mixed-field reactions. Such blood group discrepancies can either result from a haematopoiesis-confined or body-wide dispersed chimerism or mosaicism. Taking the distinct clinical consequences of these four different possibilities into account, we explored the responsible cause in nine affected individuals.

Vessel Formation Through Cross-Talk of Blood-Derived Cells and Mesenchymal Stromal Cells in the Absence of Pre-existing Vascular Structures.

Background: The generation of functional blood vessels remains a key challenge for regenerative medicine. Optimized culture set-ups mimicking the perivascular niche environment during tissue repair may provide information about the biological function and contribution of progenitor cells to postnatal vasculogenesis, thereby enhancing their therapeutic potential.

Aim: We established a fibrin-based xeno-free human 3D vascular niche model to study the interaction of mesenchymal stromal cells (MSC) with peripheral blood mononuclear cells (PBMC) including circulating progenitor cells in the absence of endothelial cells (EC), and to investigate the contribution of this cross-talk to neo-vessel formation.

Air filters for indoor environments: Interlaboratory evaluation of the new international filter testing standard ISO 16890.

Electret filters are widely used in HVAC systems to decrease particulate matter in indoor environments. The previous standard in Europe for testing air filters for general ventilation was EN 779. In July 2018, it was replaced by the new international standard ISO 16890.

Quantitative PCR of INDELs to measure donor-derived cell-free DNA-a potential method to detect acute rejection in kidney transplantation: a pilot study.

The quantification of donor-derived cell-free DNA (ddcfDNA) in recipient's plasma is a novel, but technically challenging noninvasive method to assist the diagnosis of acute rejection (AR). A quantitative real-time PCR (qPCR) approach targeting insertion/deletion polymorphisms (INDEL) was adapted to measure ddcfNA in plasma samples from 29 kidney transplant recipients obtained at time of clinically indicated biopsies (eight patients with a histologically verified AR, nine with borderline rejection and 12 without evidence of rejection). Measured ddcfDNA levels of smaller INDEL amplicon targets differed significantly (P = 0.

Prosthetic Valve Endocarditis with in a Human European Patient and Its Detection in Red Squirrels ().

Members of the genus are fastidious Gram-negative facultative intracellular bacteria that are typically transmitted by arthropod vectors. Several spp. have been found to cause culture-negative endocarditis in humans.