Light transmission of the cornea in whole human eyes.

The transmission of the cornea for light in the wavelength range 450-1000 nm was measured in steps of 50 nm by means of a photodiode implanted into the anterior chamber of whole human donor eyes. In the range from 450 nm up to 600 nm the percentage transmission was found to increase with wavelength from 80% up to 94%. In the range from 60 nm up to 1000 nm the percentage transmission was between 95% and 98%.

Photodynamic effects of hematoporphyrin-derivative on enzyme activities of murine L929 fibroblasts.

Photodynamic treatment of murine L929 fibroblasts with hematoporphyrin-derivative resulted in the inactivation of cytosolic, mitochondrial and lysosomal enzymes and in a decrease in cellular adenosine triphosphate and reduced glutathione concentrations. Comparison of these results with those of previous studies revealed that transmembrane transport systems and DNA repair enzymes are inactivated after much shorter illumination periods than are intracellular enzymes. Although the pattern of photodynamic damage altered by varying the protocol of preincubation with hematoporphyrin-derivative and washing, it appeared that under all experimental conditions the plasma membrane was much more sensitive to photodynamic damage than were the intracellular enzymes.