The mysterious case of missing lymphocytes: a cautionary tale of inter-institutional variability in outcomes of lung dissociation protocols.

Rigor and reproducibility are vital to scientific advancement. It is unclear whether a protocol optimized for tissue dissociation in one institution performs well universally. Here, we share our brand-new lab's experience with inter-institutional variability that led to the discovery that a protocol optimized for murine lung dissociation at Boston University (BU) fails to reproduce similar CD4 T cell, CD8 T cell, and B cell outcomes at the University of Michigan at Ann Arbor (U-M).

A Pipeline and Recommendations for Population and Individual Diagnostic SNP Selection in Non-Model Species.

Despite substantial reductions in the cost of sequencing over the last decade, genetic panels remain relevant due to their cost-effectiveness and flexibility across a variety of sample types. In particular, single nucleotide polymorphism (SNP) panels are increasingly favoured for conservation applications. SNP panels are often used because of their adaptability, effectiveness with low-quality samples, and cost-efficiency for population monitoring and forensics.

Mentimeter Improves Student Engagement in Online Clinical Anatomy Revision Sessions: A Programme Evaluation.

Background There is a need to develop evidence-based strategies to maximise student engagement in online learning contexts. Audience response systems (ARS) are well-established active learning tools that improve engagement in the face-to-face classroom, but they remain under-researched in the online context. Aims This study aimed to directly compare student engagement, perceptions and learning following clinical anatomy revision sessions including interactive questions asked using an ARS (Mentimeter) compared to interactive questions asked using videoconferencing interaction only.

Studying the Role of Novel Carbon Nano Tubes as a Therapeutic Agent to Treat Triple Negative Breast Cancer (TNBC) - an and Study.

Triple Negative Breast Cancer (TNBC) is a malignant cancer with a very high mortality rate around the world. African American(AA) women are 28% more likely to die from triple-negative breast cancer (TNBC) than white women with the same diagnosis. AA patients are also more likely to be diagnosed at a later stage of the disease and have the lowest survival rates for any stage of diagnosis; There are very few existing anti TNBC drugs with therapeutic efficacy hence newer anti TNBC drug design and investigation is needed.