Establishing CRISPR-Cas9 in the sexually dimorphic moss, Ceratodon purpureus.

The development of CRISPR technologies provides a powerful tool for understanding the evolution and functionality of essential biological processes. Here we demonstrate successful CRISPR-Cas9 genome editing in the dioecious moss species, Ceratodon purpureus. Using an existing selection system from the distantly related hermaphroditic moss, Physcomitrium patens, we generated knock-outs of the APT reporter gene by employing CRISPR-targeted mutagenesis under expression of native U6 snRNA promoters.

Factors Influencing the impact of nurse practitioners' clinical autonomy: a self determining perspective.

Aim: To explore factors that influence the impact of nurse practitioners' clinical autonomy with a self-determining perspective.

Background: Worldwide, there is a significant demand for healthcare professionals such as the nurse practitioner in meeting some healthcare needs across patients' lifespans. Factors influencing nurse practitioners clinical autonomy can impact the full utilisation of the role in practice.

Clinical decision-making processes among graduate nurses, specialist nurses and nurse practitioners A collaborative international study.

Aim: To explore clinical decision-making by comparing the processes used by three groups of nurses in the emergency departments of three hospitals: in Norway, Finland and Ireland.

Background: Clinical decision-making in an emergency department environment is a complex process often occurring in times of crisis. It is an important aspect contributing to the quality of care.

, an allele of , causes tissue overgrowth in the eye.

Mutant , generated via EMS mutagenesis in , was studied by undergraduate students participating in the Fly-CURE. After inducing genetically mosaic tissue in the adult eye, mutant tissue displays a robust increase in cell division and a rough appearance. Complementation mapping and sequence analysis identified a nonsense mutation in the gene , which we named ( ) due to observed increases in red-pigmented mutant tissue compared to controls.