Estimation of interaction between human keratinocytes and xenogenic collagen in vitro.

This paper describes the interaction observed between human keratinocytes and xenogenic collagen in vitro modified by HCl. Human keratinocytes were cultivated for 3-10 days, on modified and control support. Their growth, morphology and interaction with support were analyzed.

In vitro reconstruction of full thickness human skin on a composite collagen material.

Rapid progress of in vitro techniques in the last years enabled the creation of organotypic skin cultures offering new possibilities in wound treatment. Rebuilding of graft is one of the key elements of successful outcome of the procedure. In search for the best scaffold for organotypic skin culture, the novel composite xenogenic collagen based material with unique properties has been created and used to reconstitute full thickness human skin in vitro.

In vitro culture of human epithelial cells on a modified xenogenic collagen support.

Human epithelial cells (HeLa, HaCaT, NHK) were cultured in vitro on chemically modified collagen membranes. Adhesion to the support was measured by estimation of the percentage of adhering 51Cr-labeled cells. Proliferation was estimated with the XTT test.

Modification of collagen film by certain chemical agents.

Morphological changes and the content of free carboxyl groups in bovine collagen (type I) film under the influence of trypsin, hydrochloric acid (HCl) and ethylenediaminetetraacetic acid (EDTA) were studied. Incubation with trypsin and HCl was found to cause some delamination of the film and the appearance of some low-density spots. Incubation with EDTA did not cause any morphological changes.

Biological properties of preserved human dura mater grafts from different-age donors, tested in xenogenic system of transplantation.

Selected biological properties of human mater grafts obtained from different-age donors and preserved with two methods were tested in a xenogenic system of transplantation. The Regional Lymph Node Blastic Cell Reaction Test revealed relatively low immunogenicity of the material. Microscopic analysis showed that trypsinized grafts are rebuilt at a slower rate and evoke less local reaction than the ethanol-stabilized grafts.