Publications by authors named "E Lay"
Fibroblasts are the major producers of the extracellular matrix and regulate its organization. Aberrant signaling in diseases such as fibrosis and cancer can impact the deposition of the matrix proteins, which can in turn act as an adhesion scaffold and signaling reservoir promoting disease progression. To study the composition and organization of the extracellular matrix as well as its interactions with (tumor) cells, this protocol describes the generation and analysis of 3D fibroblast-derived matrices and the investigation of (tumor) cells seeded onto the 3D scaffolds by immunofluorescent imaging and cell adhesion, colony formation, migration, and invasion/transmigration assays.
View Article and Find Full Text PDFBackground: A large-scale industrial fire occurred in Rouen, France, in 2019. This study assessed the health-related quality of life of people exposed to its consequences 1 year later.
Methods: The study population comprised inhabitants of the exposed area and a non-exposed area.
Conductive polymeric microneedle (MN) arrays as biointerface materials show promise for the minimally invasive monitoring of analytes in biodevices and wearables. There is increasing interest in microneedles as electrodes for biosensing, but efforts have been limited to metallic substrates, which lack biological stability and are associated with high manufacturing costs and laborious fabrication methods, which create translational barriers. In this work, additive manufacturing, which provides the user with design flexibility and upscale manufacturing, is employed to fabricate acrylic-based microneedle devices.
View Article and Find Full Text PDFArticle Synopsis
- - TANGO2 deficiency disorder (TDD) is a rare genetic condition that leads to developmental delays, seizures, and serious metabolic and cardiac issues, and this study aimed to outline its natural history.
- - The research gathered data from 73 patients across 16 countries, revealing that most children show normal early development but experience a decline in milestones and various symptoms like ataxia and dystonia starting from ages 1 to 3.
- - Findings suggest that B-complex or multivitamin supplements can significantly reduce the occurrence of metabolic crises in these patients, offering a potential preventive measure.
Background: TANGO2 deficiency disorder (TDD) is an autosomal recessive disease associated with metabolic crisis, lethal cardiac arrhythmias, and cardiomyopathy. Data regarding treatment, management, and outcomes of cardiac manifestations of TDD are lacking.
Objective: The purpose of this study was to describe TDD-related cardiac crises.