Mechanisms underlying enhanced glycogenolysis in livers of 3,5,3'-triiodothyronine-treated rats.

Rats trained on a diurnal controlled meal-feeding schedule and injected with a single dose of 3,5,3'-triiodothyronine (T3) failed to accumulate liver glycogen and incorporated less D-[6-3H]glucose into glycogen than normally observed during the feeding period. In the experimental group, the concentration of liver adenosine 3',5'-cyclic monophosphate (cAMP) did not fall during feeding and the pattern of activities of glycogen phosphorylase, glycogen synthase, and phosphorylase kinase remained conductive to glycogenolysis. Liver lysosomal alpha-glucosidase activity normally fell during feeding periods.

Rapid presumptive identification of the Mycobacterium tuberculosis-bovis complex by radiometric determination of heat stable urease.

Simple and rapid Bactec methodologies for the determination of neat (unaltered) and heat stable urease activity of mycobacteria are presented. Clinical isolates (63) and stock cultures (32)--consisting of: M. tuberculosis (19), M.

Report of the topic study group on graduate education in medical technology.

The reported study indicates that individuals are motivated for personal reasons to obtain advanced education. Tangible benefits of more challenging employment opportunities and greater financial rewards were achieved by some of the respondents. There is apparently a deficiency in financial support of graduate education for this group of health professionals because many of the individuals reported attending graduate school on a part-time basis.

Rapid Tween 80 hydrolysis test for mycobacteria.

A rapid Tween 80 hydrolysis test for mycobacteria utilizing gas-liquid chromatography is described. The test requires 1 h of incubation followed by simple extraction and chromatography of nonderivatized oleic acid.