Molecular imaging with analyte-responsive probes offers a powerful chemical approach to studying biological processes. Many reagents for bioimaging employ a fluorescence readout, but the relatively broad emission bands of this modality and the need to alter the chemical structure of the fluorophore for different signal colors can potentially limit multiplex imaging. Here, we report a generalizable approach to multiplex analyte imaging by leveraging the comparably narrow spectral signatures of stimulated Raman scattering (SRS) in activity-based sensing (ABS) mode.
View Article and Find Full Text PDFIntroduction: This study aimed to investigate the perception thresholds of dental and gingival asymmetries in close-up smile images for orthodontists, dentists, and laypeople.
Methods: Seven sets of close-up smile images were created, in which gingival and dental asymmetries were intentionally incorporated using a software-imaging program. The alterations included unilateral changes to the gingival border and incisal edge of the central and lateral incisors and crown width of the lateral incisor.