Publications by authors named "E Kopylova"
Human skin acts as a protective barrier between the body and the external environment. Skin microbiome and intercellular lipids in the stratum corneum (SC) are essential for maintaining skin barrier function. However, the interplay between skin bacteria and the lipids is not fully understood.
View Article and Find Full Text PDFIn mammals, three genes encode IP receptors (IPRs), which are involved in agonist-induced Ca signaling in cells of apparently all types. Using the CRISPR/Cas9 approach for disruption of two out of three IPR genes in HEK-293 cells, we generated three monoclonal cell lines, IP3R1-HEK, IP3R2-HEK, and IP3R3-HEK, with the single functional isoform, IPR1, IPR2, and IPR3, respectively. All engineered cells responded to ACh with Ca transients in an "all-or-nothing" manner, suggesting that each IPR isotype was capable of mediating CICR.
View Article and Find Full Text PDFArticle Synopsis
- In 2020, researchers discovered cancer-specific microbial signals in The Cancer Genome Atlas (TCGA), leading to multiple papers confirming their findings.
- They addressed concerns about batch correction and contamination affecting results, showing that their methods yielded consistent results despite these issues.
- The development of a new method, Exhaustive, significantly improved sensitivity in data cleaning, reinforcing the validity of cancer type-specific microbial signatures in TCGA.