Publications by authors named "E Komen"
Aims: This study compared the bag-mediated filtration system (BMFS) and standard WHO two-phase separation methods for poliovirus (PV) environmental surveillance, examined factors impacting PV detection and monitored Sabin-like (SL) PV type 2 presence with withdrawal of oral polio vaccine type 2 (OPV2) in April 2016.
Methods And Results: Environmental samples were collected in Nairobi, Kenya (Sept 2015-Feb 2017), concentrated via BMFS and two-phase separation methods, then assayed using the WHO PV isolation algorithm and intratypic differentiation diagnostic screening kit. SL1, SL2 and SL3 were detected at higher rates in BMFS than two-phase samples (P < 0·05).
Article Synopsis
- - The bag-mediated filtration system (BMFS) was created to improve the environmental surveillance of poliovirus (PV) and complements traditional methods focused on acute flaccid paralysis to help with PV eradication efforts in Nairobi, Kenya, from April to September 2015.
- - During the study, environmental samples were collected and processed using two methods: BMFS (which filtered over 3 liters) and a grab sample method (collecting 1 liter). The samples were then analyzed for poliovirus presence using various techniques, including rRT-PCR.
- - Results showed that BMFS detected Sabin polioviruses more frequently than the other method, especially Sabin-like PV type 3, indicating its effectiveness for environmental monitoring, while no
Article Synopsis
- This study highlights the importance of enteric virus surveillance due to their low infectious doses and long environmental persistence, using a novel bag-mediated filtration system (BMFS).
- The researchers found that enteroviruses and PMMoV were present in 100% of wastewater samples collected from various sites in Kenya, while other viruses like adenovirus and norovirus were detected in most samples.
- The consistent presence of these viruses indicates their potential use as contamination indicators and emphasizes the need for effective monitoring of water sources worldwide.
Article Synopsis
- - Environmental surveillance of poliovirus (PV) is crucial for global eradication efforts, with the bag-mediated filtration system (BMFS) enhancing effectiveness compared to the WHO's two-phase grab method.
- - The study improved the BMFS for use in wastewater and surface waters in Nairobi by modifying aspects such as bag size and filter design to increase efficiency.
- - The enhanced BMFS can concentrate larger volumes of water (3-10 L down to 10 mL), resulting in a 6-20 times greater effective volume assayed than the WHO method, potentially reducing false-negative results and simplifying logistics for virus detection.