Action of a basic copolymer of ornithine and leucine on cells of Staphylococcus aureus.

A basic, random copolymer of l-ornithine and l-leucine (OL; molar ratios 1:1) was bactericidal to a sensitive (S) strain of Staphylococcus aureus at low concentration. Resistant cells (R) were selected from the culture medium and, after serial transfers to solutions containing increasing amounts of the polymer, grew well in the presence of very high concentrations of it (1,000 mug/ml). S cells bound much more OL than did R cells, but no difference in binding was shown between separated cell walls or cell membranes of S and R.

Transformation of neuraminidase-treated lymphocytes by soybean agglutinin.

Transformation of mouse-spleen lymphocytes in the presence of soybean agglutinin is markedly enhanced after their treatment with neuraminidase (EC 3.2.1.

Induction of lymphocyte transformation by sequential treatment with neuraminidase and galactose oxidase.

Treatment of mouse-spleen cells with galactose oxidase (EC 1.1.3.