Publications by authors named "E Kalef"
Article Synopsis
- Phosphorylation of Mdm2 in response to DNA damage prevents the degradation of p53 and reduces its interaction with the monoclonal antibody (mAb) 2A10.
- Researchers identified two specific epitopes on human Mdm2 (positions 255-266 and 389-400) using a phage-peptide library, which can inhibit the binding of mAb2A10 with high and moderate affinity.
- Phosphorylated peptides and specific Mdm2 mutations significantly decrease the binding affinity with mAb2A10, explaining how phosphorylation affects Mdm2's reactivity with the antibody.
Calcium-dependent oxidation of thioredoxin during cellular growth initiation.
Biochem Biophys Res Commun
January 2002
The fraction of cell thiol proteins in the oxidized disulfide form were quantified during mitogen-induced HaCaT keratinocyte growth initiation. Oxidized thioredoxin increased from 11 +/- 1.2% in resting cells to 80 and 61% 2 min after addition of bradykinin or EGF.
View Article and Find Full Text PDFEmploying a combinatorial phage-peptide library, we previously identified the peptide MRYYESSLKSYPD (designated, library-peptide) that binds the snake toxin alpha-bungarotoxin (alpha-BTX) with a moderate binding constant of 10(-6)M (Balass et al., 1997. Proc.
View Article and Find Full Text PDFSome 5% of the soluble proteins of L1210 murine leukemia lymphoblasts contain surface vicinal thiols (Kalef, E., Walfish, P. G.
View Article and Find Full Text PDFUse of cationic detergents to enhance reactivity of protein sulfhydryls.
Methods Enzymol
September 1995