Publications by authors named "E K Brundage"
Background: A healthy heart is able to modify its function and increase relaxation through post-translational modifications of myofilament proteins. While there are known examples of serine/threonine kinases directly phosphorylating myofilament proteins to modify heart function, the roles of tyrosine (Y) phosphorylation to directly modify heart function have not been demonstrated. The myofilament protein TnI (troponin I) is the inhibitory subunit of the troponin complex and is a key regulator of cardiac contraction and relaxation.
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- Troponin I (TnI) is crucial for heart contraction and relaxation, with its phosphorylation at Ser-23/24 being important for heart function regulation.
- * Research on mice with TnI mutated at Ser-23/24 showed that without phosphorylation, there were notable impairments in heart function under both normal and stressed conditions.
- * Mice lacking TnI Ser-23/24 phosphorylation exhibited worse heart performance, less ability to respond to increased heart rates, and a poorer response to stress, indicating the significance of this phosphorylation site for cardiac health.
Article Synopsis
- Phosphorylation and acetylation of β-myosin heavy chain (β-MHC) are key processes that affect heart muscle contraction, with new research identifying specific post-translational modifications (PTMs) in both healthy and failing human hearts.
- The study found significant modifications including six acetylated lysines and two phosphorylated residues, particularly noting that the acetylation at K951 was significantly reduced in failing hearts, which may affect the stability and function of muscle fibers.
- Additionally, these modifications could influence the interactions between β-MHC and other proteins, ultimately impacting heart muscle contractility and the mechanics of heart failure, highlighting the importance of PTM location over heart disease type.
This study measured how heart failure affects the contractile properties of the human myocardium from the left and right ventricles. The data showed that maximum force and maximum power were reduced by approximately 30% in multicellular preparations from both ventricles, possibly because of ventricular remodeling (e.g.
View Article and Find Full Text PDFWe report the design and operation of a surface-emitting surface acoustic wave (SAW) acousto-optical modulator which behaves as a cm-scale linear hologram in response to an applied electronic waveform. The modulator is formed by an optical waveguide, transducer, and out-coupling surface grating on a 1 mm-thick lithium niobate substrate. We demonstrate the ability to load and illuminate a 9-region linear hologram into the modulator's 8 mm-long interaction region using applied waveforms of 280-320 MHz.
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