Determination of non-protein bound phenylbutazone in bovine plasma using ultrafiltration and liquid chromatography with ultraviolet detection.

A liquid chromatographic procedure using UV detection was coupled with ultrafiltration for the quantitation of free phenylbutazone in bovine plasma, in the range of 20 ng/ml to 2.0 microg/ml. Whole plasma samples (0.

Pharmacokinetics of phenylbutazone in plasma and milk of lactating dairy cows.

Phenylbutazone was administered intravenously (i.v.) to a group of four lactating cows at a dosage of 6 mg/kg body weight.

Determination of phenylbutazone residues in bovine milk by liquid chromatography with UV detection.

A published liquid chromatographic (LC) method was modified for quantitation of phenylbutazone (PBZ) residues in the range of 25-300 ng/mL in bovine milk. Milk samples (1 mL) were diluted with absolute ethanol and 25% NH4OH. Diethyl ether and petroleum ether were added sequentially to the milk extract and the mixture was agitated on a Vortex mixer to partition out milk fat.

Photosynthate metabolism in the source leaves of n(2)-fixing soybean plants.

Soybean plants (Glycine max [L.] Merr. cv Williams), which were symbiotic with Bradyrhizobium japonicum, and which grew well upon reduced nitrogen supplied solely through N(2) fixation processes, often exhibited excess accumulation of starch and sucrose and diminished soluble protein in their source leaves.

Photosynthesis and photosynthate partitioning in n(2)-fixing soybeans.

Leaf area, chlorophyll content, net CO(2) photoassimilation, and the partitioning of fixed carbon between leaf sucrose and starch and soluble protein were examined in Glycine max (L) Merr. cv Williams grown under three different nitrogen regimes. One group (Nod+/+) was inoculated with Bradyrhizobium and watered daily with a nutrient solution containing 6 millimolar NH(4)NO(3).