Protocol for assessing phagocytosis activity in cultured primary murine microglia.

In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we describe treatment with lipopolysaccharide (LPS) to induce phagocytosis of fluorescent beads, followed by quantitative analysis using fluorescent imaging and Fiji - ImageJ software.

Food Insecurity and Childhood Obesity: A Systematic Review.

Background And Objectives: Addressing food insecurity while promoting healthy body weights among children is a major public health challenge. Our objective is to examine longitudinal associations between food insecurity and obesity in US children aged 1 to 19 years.

Methods: Sources for this research include PubMed, CINAHL, and Scopus databases (January 2000 to February 2022).

Decolonizing agriculture in the United States: Centering the knowledges of women and people of color to support relational farming practices.

While the agricultural knowledges and practices of Black, Indigenous, and People of Color (BIPOC) and women have shaped agriculture in the US, these knowledges have been colonized, exploited, and appropriated, cleaving space for the presently dominant white male agricultural narrative. Simultaneously, these knowledges and practices have been transformed to fit within a society that values individualism, production, efficiency, and profit. The authors use a decolonial Feminist Political Ecology framework to highlight the ways in which the knowledges of Indigenous, Black, and women farmers have been and are being colonized; a tradition that makes alternative agriculture a predominantly white space.

Ethical Issues and the Electronic Health Record.

Ethical issues related to electronic health records (EHRs) confront health personnel. Electronic health records create conflict among several ethical principals. Electronic health records may represent beneficence because they are alleged to increase access to health care, improve the quality of care and health, and decrease costs.

Thirty-Day Hospital Readmissions: A Predictor of Higher All-cause Mortality for Up to Two Years.

Introduction Readmission within 30 days is used as a standard quality metric for hospitalized patients. We hypothesized that patients who get readmitted within 30 days may have higher short- and long-term mortality. Material and Methods Using administrative data, we retrospectively analyzed 2,353 patients admitted to inpatient medicine service over a period of one year.