Endothelialectomy With Trypan Blue Staining to Enhance Descemetorhexis Visualization.

Purpose: To describe a technique involving combined endothelialectomy and trypan blue staining to allow for improved visualization and Descemet membrane (DM) removal during endothelial keratoplasty.

Methods: Endothelialectomy with 2 disposable endothelial irrigating cannulas (Vortex and Sterimedix) and an irrigation-aspiration handpiece are described. Several passes over the desired area are made to ensure adequate endothelialectomy treatment.

Cohort profile: creation of the SAIL MELD-B e-cohort (SMC) and SAIL MELD-B children and young adult e-cohort (SMYC) to investigate the lived experience of the 'burdensomeness' of multimorbidity.

Purpose: We have established the SAIL MELD-B electronic cohort (e-cohort SMC) and the SAIL MELD-B children and Young adults e-cohort (SMYC) as a part of the Multidisciplinary Ecosystem to study Lifecourse Determinants and Prevention of Early-onset Burdensome Multimorbidity (MELD-B) project. Each cohort has been created to investigate and develop a deeper understanding of the lived experience of the 'burdensomeness' of multimorbidity by identifying new clusters of burdensomeness concepts, exploring early life risk factors of multimorbidity and modelling hypothetical prevention scenarios.

Participants: The SMC and SMYC are longitudinal e-cohorts created from routinely collected individual-level population-scale anonymised data sources available within the Secure Anonymised Information Linkage (SAIL) Databank.

DNAJC13 localization to endosomes is opposed by its J domain and its disordered C- terminal tail.

Unlabelled: Endosomes are a central sorting hub for membrane cargos. DNAJC13/RME-8 plays a critical role in endosomal trafficking by regulating the endosomal recycling or degradative pathways. DNAJC13 localizes to endosomes through its N-terminal Plekstrin Homology (PH)-like domain, which directly binds endosomal phosphoinositol-3-phosphate (PI(3)P).

Community Synchrony in Seed Production is Associated With Trait Similarity and Climate Across North America.

Mast seeding, the synchronous and highly variable production of seed crops by perennial plants, is a population-level phenomenon and has cascading effects in ecosystems. Mast seeding studies are typically conducted at the population/species level. Much less is known about synchrony in mast seeding between species because the necessary long-term data are rarely available.

Exploring the Laws of Developmental Direction Using a Documented Skeletal Collection.

Objectives: Many human growth studies note a trend of differential variation in limb segment lengths, where distal elements show greater variability than their proximal counterparts. This has been attributed to their developmental sequence, where bones further from the head develop later and are more impacted by fluctuating growth conditions. We aimed to explore limb dimensions within this framework, known as the laws of developmental direction, in children (0.