[Free-radical reactions in Daphnia after low doses of γ-irradiation in vivo].

A violation of free-radical reactions has been found by the method of free-radical copolymerization in the two groups of Daphnia magna exposed to 100 and 1000 mGy of gamma-rays and their non-exposed first generation progeny. The method is based on a quantitative radiometric registration of the polymerization process that develops in the cells of a multicellular organism in tissue in proportion to the existing number of free radicals. The fact of the increased level of free radicals may be indicative of the hereditary transgenerational effect and the cause of lower survival of Daphnia in both generations.

[Methyltetrazolium bromide (MTT) was applied for bioassay of environmental radioactivity in vivo].

The MTT-assay is a colorimetric assay that measures the activity of enzymes that reduce MTT (a yellow tetrazolium bromide) in living cells. The MTT-test has been traditionally applied for the analysis of drug cytotoxicity in vitro. In our study MTT-assay was first applied for the investigation in vivo of the mechanisms of non-targeted effects of radiation and development of stress in multicellular crustaceans Daphnia magna.

[The change of Daphnia magna viability after low doses of gamma-irradiation].

The gamma-irradiation in the dose 0.001 Gy didn't influence to the Daphnia magna Straus survival in the parents generation, cultivating during 30 days. At the some time the irradiation in dose 0.

[Spontaneous motional activity of paramecium Spirostomum ambiguum after gamma-irradiation in a broad variety of dose as a bioassay].

The spontaneous motional activity of paramecium Spirostomum ambiguum Ehrbg. has high sensitivity to the gamma-irradiation. The increase of this parameter has been observed just after irradiation in the 0.