Single-cell phenotyping of extracellular electron transfer via microdroplet encapsulation.

Electroactive organisms contribute to metal cycling, pollutant removal, and other redox-driven environmental processes via extracellular electron transfer (EET). Unfortunately, developing genotype-phenotype relationships for electroactive organisms is challenging because EET is necessarily removed from the cell of origin. Microdroplet emulsions, which encapsulate individual cells in aqueous droplets, have been used to study a variety of extracellular phenotypes but have not been applied to investigate EET.

The effect of the biohydrogenation-induced milk fat depression in dairy cows on milk odd and branched chain fatty acids across multiple studies.

Diet-induced milk fat depression (MFD) caused by UFA, and low fiber diets results in an increase in alternate rumen biohydrogenation intermediates. The impact of these MFD-inducing diets on milk odd and branched chain fatty acids (OBCFA) is not well known. The first objective of this study was to characterize the time course of changes in OBCFA in milk fat during induction and recovery of MFD induced with a high UFA and low fiber diet in 3 separate experiments.

A field resource for the glioma cerebrospinal fluid proteome: impacts of resection and location on biomarker discovery.

Background: While serial sampling of glioma tissue is rarely performed prior to recurrence, cerebrospinal fluid (CSF) is an underutilized longitudinal source of candidate glioma biomarkers for understanding therapeutic impacts. However, the impact of key variables to consider in longitudinal CSF samples for monitoring biomarker discovery, including anatomical location and post-surgical changes, remains unknown.

Methods: Aptamer-based proteomics was performed on 147 CSF samples from 74 patients, 71 of whom had grade 2-4 astrocytomas or grade 2-3 oligodendrogliomas.

Integrated Care for People Living With Rare Disease: A Scoping Review on Primary Care Models in Organization for Economic Cooperation and Development Countries.

Introduction/objectives: Individually rare, rare diseases are collectively common resulting in frequent health system use. Navigating the health system persists as a challenge. Primary care provides longitudinal contact with the health system and is placed to provide integrated rare-disease-care.