Angiogenesis in rat uterine cicatrix after injection of autologous bone marrow mesenchymal stem cells.

Results of injection of autologous bone marrow mesenchymal stem cells with transfected GFP gene into the rat uterine horn cicatrix were studied by light microscopy. Large groups of blood vessels with blood cells inside were seen after injection of autologous bone marrow cells into the cicatrix on the right horn, formed 2 months after its ligation; no groups of vessels of this kind were found in the cicatrix in the contralateral horn. Examination of unstained sections in reflected UV light showed sufficiently bright fluorescence in the endothelium and outer vascular membrane in the uterine horn cicatrix only on the side of injection.

[Morphological analysis of the results of injection of the autologous stromal marrow-derived stem cells into the uterine scar in rats].

The results of injection of the autologous stromal (mesenchymal) marrow-derived stem cells (ASMSC) transfected with green fluorescent protein (GFP) gene into the rat uterine scar were studied by methods of light microscopy. One week after ASMSC injection into the formed scar on the right side (2 months after the uterine horn ligation), large groups of blood vessels containing the formed elements of blood were found, while these were absent in the uterine scar on the opposite side. The study of the unstained sections in the reflected ultraviolet light demonstrated bright luminescence in the vessel endothelium and adventitia in the uterine horn scar only at the side of ASMSC injection.

[Absorbable suture material in the body].

Light microscopy was used to study postcesarean section uterine scars removed at resurgery and rat urine tissues after myometrial scar simulation. The myometrial scar tissue of 1 of 30 patients and 4 of 42 experimental animals exhibited vicryl suture material remains surrounded by a connective tissue capsule and, frequently, infiltrated leukocytes and giant cells of foreign bodies. It is not inconceivable that the presence of these foreign bodies in the tissues of the uterus and its scar may lower the strength of the wall of this organ during pregnancy and labor.