[An evaluation of the allergizing properties of merthiolate as a component of adsorbed DTP vaccine].

The study of the allergenic properties of thimerosal contained in adsorbed DPT vaccine was carried out. Two variants of this vaccine, with and without thimerosal, as well as thimerosal alone (diluted 1:10,000, which corresponds to the content of this preservative in the vaccine), were used in experiments. The phenomenon of anaphylactic shock, passive dermal anaphylaxis and skin tests were used as test-systems.

[A comparative immunochemical analysis of allergoids and allergens].

In comparison with allergens having protein fragments with a molecular weight not exceeding 110 kD, allergoids have been found to consist of larger fragments with a molecular weight of 10-150 kD. Allergoids have less charged components than initial allergens and less antigenic components. Allergoids retain their capacity for stimulating the production of antibodies, specific to all antigenic components.

[A comparative evaluation of the biological action of allergens and allergoids prepared from plant pollen by the double radial immunodiffusion method].

Antisera to allergens and allergoids prepared from timothy, orchard grass, birch and wormwood pollen have been obtained and used in the double radial immunodiffusion test. The preparations of the allergoid row have been found capable of inducing immune response in laboratory animals (rabbits). Both forms of pollen preparations, allergens and allergoids, have been shown to possess common antigenic determinants reacting with antibodies present in antisera to allergens and allergoids.

[Use of isoelectric focusing for assessing the composition of pollen allergens].

The preparations of allergens and allergoids obtained from ragweed, timothy and wormwood pollen, as well as the preparations of allergens from birch and orchard grass pollen differing in the method of their production, have been studied with the use of analytical isoelectric focusing in a thin gel layer. The composition of the preparations of allergoids differs from that of the allergenic preparations from the pollen of the same plant species by the decreased content of protein components detected in this investigation. The main proteins contained in the preparations of allergoids are distributed in the zone of pH 3.