Allelic Variants of Gene in Isolates from Patients with Ixodid Tick-Borne Borreliosis.

Protein P66 is one of the crucial virulence factors of , inducing the production of specific antibodies in patients with ixodid tick-borne borreliosis (ITBB). Various species of are characterized by genetic variability of the surface-exposed loop of P66. However, little is known about this variability in Here we describe the variability of the nucleotide sequences of gene locus in isolates of Analysis of nucleotide sequences of P66 in 27 isolates of from ITBB patients revealed three allelic variants of this gene.

Utility of sensu stricto C6 Peptide for Serologic Confirmation of Erythema-Free Ixodid Tick-Borne Borrelioses in Russia.

We evaluated the utility of sensu stricto peptide C6 for serologic confirmation of Ixodid Tick-Borne Borrelioses (ITBB) in Russia. Serum samples (N = 1089) were from erythema migrans (EM) (N = 327) and the EM-free (EMF) patients (N = 115); in some patients, the disease was accompanied by human granulocytic anaplasmosis or tick-borne encephalitis. The sera were investigated by multiplex phosphorescence analysis (PHOSPHAN) for IgM to C6, recombinant OspC and VlsE proteins, and IgG to C6 from , (), and ().

FIRST MOLECULAR IDENTIFICATION OF THE TULAREMIA AGENT IN THE TICKS IXODES TRIANGULICEPS BIR. IN RUSSIA.

The ticks Ixodes trianguliceps (140 nymph pool and 211 adults) collected from small forest mammals in the forests of the Middle Urals (Chusovskoy district of the Perm Region) were tested using real-time PCR for the presence of Francisella tularensis DNA. Using the target gene 16S rRNA, the locus size 1165-1170 bp Francisella DNA was detected in 12 adults and 4 pools of nymphs. DNA-positive samples from 17 individuals from 128 adults and in 16 of 89 nymph pools were additionally detected by amplification of a shorter locus of the same gene (221-222 bp).

C6 Peptide-Based Multiplex Phosphorescence Analysis (PHOSPHAN) for Serologic Confirmation of Lyme Borreliosis.

Background: A single-tier immunoassay using the C6 peptide of VlsE (C6) from Borrelia burgdorferi sensu stricto (Bb) has been proposed as a potential alternative to conventional two-tier testing for the serologic diagnosis of Lyme disease in the United States and Europe.

Objective: To evaluate the performance of C6 peptide based multiplex Phosphorescence Analysis (PHOSPHAN) for the serologic confirmation of Lyme borreliosis (LB) in Russian patients.

Methods: Serum samples (n = 351) were collected from 146 patients with erythema migrans (EM); samples from 131 of these patients were taken several times prior to treatment and at different stages of recovery.