Technical Strategy of Bone Graft Augmentation in the Anterior Maxilla.

Bone graft augmentation in the anterior maxilla has a high successful rate. However, suture dehiscence and consequent bone graft exposure can compromise and fail this procedure. Therefore, this article presents a new strategy of closure technique to guarantee the bone graft augmentation.

Interferon immunogenicity: technical evaluation of interferon-alpha 2a.

Observations from some studies with interferon-alpha 2a (IFN-alpha 2a) have shown the presence of neutralizing antibodies in a proportion of patients. As a result, an investigation into the production of antibodies to IFN-alpha 2a was undertaken. A number of technical aspects of its production and storage were investigated, including the possibility of an incorrect structure, which could affect the immunogenicity of the IFN-alpha 2a molecule.

[Possibilities of gene-technological production of drugs, especially also the interferons].

For a long time proteins have been used in Medicine as medication. Human Growth Hormone (isolated from Pituitaries of Cadavers) or insuline (isolated from porcine or bovine pancreas) are examples. Genetic engineering has essentially simplified the production of such well known proteins and on the other hand enabled search for and characterization of new human proteins.

Immobilization of beta-galactosidase for application in organic chemistry using a chelating peptide.

The strong interaction of hexa-histidine fusion proteins with metal chelate adsorbents was utilized to immobilize beta-galactosidase with a hexa-histidine peptide at the N-terminus to the Ni(2+)-nitrilotriacetic acid adsorbent. The fusion protein was cloned and expressed in Escherichia coli. The purified soluble fusion protein showed the same specific activity as the purified beta-galactosidase and retained 64 percent of its beta-galactosidase activity when bound to the adsorbent.