Background: Although fecal microbiota transplant has been used to prevent recurrent Clostridioides difficile infection (rCDI), documented pathogen transmissions highlight inherent safety risks of minimally processed stool. We describe manufacturing processes for fecal microbiota spores, live (VOWST; VOS, formerly SER-109), a microbiota-based oral therapeutic of Firmicutes spores.
Methods: Bacterial inactivation kill curves were obtained after ethanol exposure for 4 model organisms spiked into process intermediates.
The main challenge addressed in this paper is how to handle and recycle the large amount of C&D waste that is generated from infrastructure projects. The study is motivated by Bærum Ressursbank in Norway and their aim of finding logistical solutions to an expected surplus of 15 million m of waste from infrastructure projects in the next decade. We identify the key decisions as the design of the distribution network for both surplus waste materials and new construction materials and the investments in processing machinery at each recycling facility, and we call the problem representing this situation the Infrastructure Waste Management Problem (IWMP).
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