Cholesterol distribution in renal epithelial cells LLC-PK1 as determined by cholesterol oxidase: evidence that glutaraldehyde fixation masks plasma membrane cholesterol pools.

Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush border membranes isolated from the apical domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B.

Effect of cholesterol oxidase treatment on physical state of renal brush border membranes: evidence for a cholesterol pool interacting weakly with membrane lipids.

Treatment with cholesterol oxidases has shown that cholesterol is heterogeneously distributed in brush borders isolated from the apical membrane domain of the renal and intestinal epithelial cells [Bloj, B., & Zilversmit, D. B.

Sphingomyelin and cholesterol modulate sodium coupled uptakes in proximal tubular cells.

Sphingomyelin (SM) and cholesterol are major lipid species of apical membranes in renal proximal tubular cells and confer to these membranes a low fluidity. Changes in membrane fluidity and/or lipidic composition were shown to affect the activity of cotransport systems of renal apical membranes. We evaluated the effect of decreasing membrane SM content on lipidic composition, membrane fluidity and sodium (Na)coupled uptakes in rabbit proximal tubular cells in primary culture.