Public holidays, school holidays, and long weekends: Effects on drowning rates in New South Wales.

Objective: To quantify drowning rates and fatal to non-fatal drowning ratios on public holidays, school holidays, weekdays and long weekends in New South Wales from January 2010 to June 2022.

Methods: Using a linked administrative dataset comprising ambulance (paper-based and electronic records), emergency department presentations and death registry, rates of drowning and ratios of fatal to non-fatal drowning were calculated.

Results: Across 4,161 total drowning incidents, public holidays (14.

Optimized breath analysis: customized analytical methods and enhanced workflow for broader detection of VOCs.

Introduction: Breath Volatile organic compounds (VOCs) are promising biomarkers for clinical purposes due to their unique properties. Translation of VOC biomarkers into the clinic depends on identification and validation: a challenge requiring collaboration, well-established protocols, and cross-comparison of data. Previously, we developed a breath collection and analysis method, resulting in 148 breath-borne VOCs identified.

Epigenetic phase variation in the gut microbiome enhances bacterial adaptation.

The human gut microbiome within the gastrointestinal tract continuously adapts to variations in diet, medications, and host physiology. A central strategy for genetic adaptation is epigenetic phase variation (ePV) mediated by bacterial DNA methylation, which can regulate gene expression, enhance clonal heterogeneity, and enable a single bacterial strain to exhibit variable phenotypic states. Genome-wide and site-specific ePV have been well characterized in human pathogens' antigenic variation and virulence factor production.

Structural Dynamics of the Ubiquitin Specific Protease USP30 in Complex with a Cyanopyrrolidine-Containing Covalent Inhibitor.

Inhibition of the mitochondrial deubiquitinating (DUB) enzyme USP30 is neuroprotective and presents therapeutic opportunities for the treatment of idiopathic Parkinson's disease and mitophagy-related disorders. We integrated structural and quantitative proteomics with biochemical assays to decipher the mode of action of covalent USP30 inhibition by a small-molecule containing a cyanopyrrolidine reactive group, . The inhibitor demonstrated high potency and selectivity for endogenous USP30 in neuroblastoma cells.