The role of automation etiquette and task-criticality on performance, workload, automation reliance, and user confidence.

Previous research suggests good automation etiquette can yield positive effects on user performance, trust, automation reliance, and user confidence - especially in personified or anthropomorphized technologies. The current study examined the impact of automation etiquette and task-criticality in non-personified technology. The study used a computer-based automation task to examine good and bad automation etiquette models and different domain-based task-criticality levels (between-subjects) that contained various stages of automation (stage 2 and stage 3) and automation reliability levels (60% and 80%) (within-subjects).

Polite AI mitigates user susceptibility to AI hallucinations.

With their increased capability, AI-based chatbots have become increasingly popular tools to help users answer complex queries. However, these chatbots may hallucinate, or generate incorrect but very plausible-sounding information, more frequently than previously thought. Thus, it is crucial to examine strategies to mitigate human susceptibility to hallucinated output.

Targeting PD-1 T cells with small-format immunocytokines enhances IL-12 antitumor activity.

Immunostimulatory cytokines and immune checkpoint inhibitors hold promise as cancer therapeutics; however, their use is often limited by reduced efficacy and significant toxicity. In this study, we developed small-format immunocytokines (ICKs) based on interleukin-12 (IL-12) and blocking nanobodies (Nbs) targeting mouse and human programmed cell death 1 (PD-1) and programmed cell death ligand 1 (PD-L1). Both PD-1- and PD-L1-targeted ICKs demonstrated similar in vitro performance, significantly increasing IL-12 tethering to immune cells and enhancing T cell cytotoxic activity compared with IL-12 alone.

Engineering U1-Based Tetracycline-Inducible Riboswitches to Control Gene Expression in Mammals.

Synthetic riboswitches are promising regulatory devices due to their small size, lack of immunogenicity, and ability to fine-tune gene expression in the absence of exogenous trans-acting factors. Based on a gene inhibitory system developed at our lab, termed U1snRNP interference (U1i), we developed tetracycline (TC)-inducible riboswitches that modulate mRNA polyadenylation through selective U1 snRNP recruitment. First, we engineered different TC-U1i riboswitches, which repress gene expression unless TC is added, leading to inductions of gene expression of 3-to-4-fold.

U1A is a positive regulator of the expression of heterologous and cellular genes involved in cell proliferation and migration.

Here, we show that direct recruitment of U1A to target transcripts can increase gene expression. This is a new regulatory role, in addition to previous knowledge showing that U1A decreases the levels of U1A mRNA and other specific targets. In fact, genome-wide, U1A more often increases rather than represses gene expression and many U1A-upregulated transcripts are directly bound by U1A according to individual nucleotide resolution crosslinking and immunoprecipitation (iCLIP) studies.