Allele frequencies of human platelet antigen 1, 2, 3, and 5 systems in patients with chronic refractory autoimmune thrombocytopenia and in normal persons.

Background And Objectives: Chronic refractory autoimmune thrombocytopenic purpura (AITP) is an autoimmune disorder due to autoantibodies against platelet glycoproteins (GP). Human platelet alloantigenic (HPA) systems are distributed to different platelet GPs. We carried out genotyping of diallelic HPA-1, -2, -3, and -5 systems to clarify potential associations between HPA alleles and the development of chronic refractory AITP.

[Thrombomatch--an ELISA test for detection of antibodies to HLA antigens and platelet-specific antigens. Clinical experiences in compatibility testing of platelet transfusions].

We tested a new ELISA--called thrombomatch--for antibody detection against HLA- and platelet-specific antigens in comparison with MAIPA, Capture-P-Modified an lymphocytotoxicity. Sensitivity of the thrombomatch ist a little lower than the MAIPA, higher than lymphocytotoxicity and Capture-PM in the alloantibody detection. The thrombomatch is very helpful for a immunological monitoring of the antibody formation while long-term blood substitution in hemato-oncological patients.