Publications by authors named "E Garner"
Biosolids can provide a nutrient rich soil amendment, particularly for poor soils and semi-arid or drought-prone areas. However, there are concerns that sludge and biosolids could be a source of propagation and exposure to AMR determinants such as antibiotic resistant bacteria (ARB), and antibiotic resistance genes (ARGs). To inform risk assessment efforts, a systematic literature review was performed to build a comprehensive spreadsheet database of ARB and ARG concentrations in biosolids (and some sludges specified as intended for land application), along with 69 other quantitative and qualitative meta-data fields from 68 published studies describing sampling information and processing methods that can be used for modeling purposes.
View Article and Find Full Text PDFAs water reuse applications expand, there is a need for more comprehensive means to assess water quality. Microbiome analysis could provide the ability to supplement fecal indicators and pathogen profiling toward defining a "healthy" drinking water microbiota while also providing insight into the impact of treatment and distribution. Here, we utilized 16S rRNA gene amplicon sequencing to identify signature features in the composition of microbiota across a wide spectrum of water types (potable conventional, potable reuse, and nonpotable reuse).
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- The study explored the behavior of antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs) and found significant variation in ARG responses, as they may either decrease or increase depending on the specific plant.
- Researchers used metagenomic sequencing on samples from 12 international WWTPs, identifying 1079 different ARGs, with overall decreases in ARG abundance observed at most sites, except for one where 40% of ARGs increased.
- The findings suggest that while WWTPs generally reduce antibiotic resistance loads, the increase in mobile genetic elements during treatment does not necessarily correlate with more transmissible ARGs, indicating a need for better wastewater surveillance strategies.
Eukaryotic cells have been evolving for billions of years, giving rise to wildly diverse cell forms and functions. Despite their variability, all eukaryotic cells share key hallmarks, including membrane-bound organelles, heavily regulated cytoskeletal networks and complex signaling cascades. Because the actin cytoskeleton interfaces with each of these features, understanding how it evolved and diversified across eukaryotic phyla is essential to understanding the evolution and diversification of eukaryotic cells themselves.
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