[The viral genome status studied under the conditions of a mixed infection in lymphoblastoid cells by adenovirus and the Epstein-Barr virus].

Some indices have been studied which characterized the state of Epstein-Barr virus genome and adenovirus in the implanted lines of lymphoblastoid cells of B and T phenotype under the mixed or monoinfection. It has been shown that super infection by type 2 adenovirus rather sharply affects the state of Epstein-Barr virus genome in the Raji cells containing integrated Epstein-Barr virus genome. The state of adenovirus genome in the studied cells is less subject to changes.

[Genomic disorders in the mononuclear blood cells of those who worked in the cleanup of the accident at the Chernobyl Atomic Electric Power Station].

The results of molecular investigations of blood mononuclears from 120 clean-up workers after 7-9 years of Chernobyl accident with the total exposure radiation doses ranging from 5 to 76 cGr are presented. Structural polymorphism of the leukemia associated bcr and ribosomal RNA (rRNA) genes were studied using Southern blot hybridization. Allelic polymorphism of bcr gene with characteristic for leukemia allele distribution was detected in 16.

[Expression and function of x-mla genes associated with leukemic transformation of cells].

Expression of genes, associated with leukemic transformation and called x-mla, was studied in cells of various types of experimental leukemia and in homologous normal cells. Augmentation of x-mla expression in malignant haemopoietic cells and lack or very low expression in normal tissues and non-haemopoietic tumors of mice and rats has been revealed. The involving of x-mla in proliferation control of leukemic but not normal cells has been shown.

[Effect of histone H5 on RNA synthesis in leukemic cells and on the translation process in vitro].

The effects of the erythrocyte-specific histone H5 from chicken erythrocytes on HnRNA synthesis in splenocytes of Rauscher virus-infected mice were studied. It was shown that histone H5 represses the synthesis of 35S RNA typical of the Rauscher virus-producing cells but does not influence the synthesis of other HnRNA fractions. The effects of H5 and other histones on the effectiveness of poly(U) translation in a cell-free system of wheat embryos were investigated.

[Effect of histone H5 on the development of virus-induced leukemia and RNA synthesis in leukemic cells].

Development of virus-induced Rauscher leukosis was studied after administration into mice of histone H5 isolated from chicken erythrocyte nuclei by means of gel filtration through Sephadex G-75. Histone H5, administered intraperitoneally at a dose of 100 mg/kg into mice treated with Rauscher virus, inhibited the leukosis development (T = 29.7%).