Extracellular proteins of goldfish optic tectum labeled by intraocular injection of 3H-proline.

A prominent group of soluble glycoproteins with a molecular weight of 30K-40K and pI 5.0-5.6 was detected in various parts of the goldfish brain as well as in the optic nerves.

Recent advances in the purification, characterization and structure determination of lipases.

Recently, lipases have been purified from mammalian, bacterial, fungal and plant sources by different methodologies. Purified lipases subsequently have been characterized for molecular size, metal binding capabilities, glycoside and phosphorus contents, and substrate specificities. Primary structures of several lipases have been determined either from amino acid or nucleic acid sequences.

The high-molecular-weight proteins of bovine brain plain synaptic vesicles.

High molecular mass polypeptides (Mr greater than 100,000) of plain synaptic vesicles from bovine cerebral cortex were separated using porous polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Four major bands, of Mr 262,000, 249,000, 216,000, and 173,000, were resolved. Investigations into the membrane association of the Mr 216,000 and 173,000 proteins by means of solubilization experiments and Sepharose 4B chromatography indicate that the former is a peripheral protein and the latter is more firmly attached, possibly an integral protein.

Fast axonally transported proteins in regenerating goldfish optic nerve: effect of abolishing electrophysiological activity with TTX.

Blocking neural activity with intraocular tetrodotoxin (TTX) hinders regeneration of goldfish optic axons, and prevents the refinement of the retinotopic map that is formed in the optic tectum. The latter effect is not observed with TTX treatment confined to the first two weeks of regeneration, but is produced when the TTX treatment is delayed until after this time. In the present study, 2-dimensional gel electrophoresis was used to analyse the effects of two different schedules of TTX treatment (0-9 days or 14-32 days) on incorporation of [3H]proline into individual proteins conveyed by fast axonal transport in the optic nerve.